Friday, May 12, 2023     1:45 PM – 2:30 PM ET     Exhibit Hall D, Room 1

Exhibitor Workshop: Thermo Fisher Scientific

Ventures Into the Fluorosphere—What Spectral Cytometry Teaches Us About Conventional Cytometry and the Everchanging Topography of the Immunological Landscape

Presenter

• Kim Lueck, R&D Scientist, Protein & Cell Analysis

High dimensional full spectrum flow cytometry grants access to previously unattainable parameters in cellular immunology. However, spectral cytometry comes with its own idiosyncrasies and unique constraints. In this tutorial, we take a deep dive into a broadly focused human immunophenotyping panel with over 30 parameters to highlight some of the special features and potential pitfalls of spectral panel design. A demonstration of how such a panel can be adapted to deeply focus on specific immune cell subpopulations will also be presented.